Role of de novo DNA methyltransferases in hematopoietic stem cells (RNA-seq)
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=9101bb182a339b4cf8cd612dcd7771f9
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To investigate the global transcriptome expression in Dnmt3ab dKO HSCs, we performed high-throughput sequencing of Poly A+ RNA (RNA-Seq) from purified HSCs (SP-KSL-CD150+). With biological duplicates, more than 200 million reads in total for each genotype of HSC were obtained.
为探究Dnmt3ab双敲除造血干细胞（Hematopoietic Stem Cells，HSCs）中的全局转录组表达水平，我们对分选获得的SP-KSL-CD150+造血干细胞开展了Poly A+ RNA高通量测序（RNA-Seq）。本实验设置生物学重复，每种基因型的造血干细胞总计获得超过2亿条测序读段。
提供机构：
Texas A&M University
创建时间：
2022-02-20
