Identification of genes involved in CEACAM1-4S mediated lumen formation in a 3D model of mammary morphogenesis. Homo sapiens

Identification of genes involved in lumen formation: gene chip analysis was performed on mRNA isolated from both wild type and T457A,S459A mutated CEACAM1-4S transfected MCF7 cells grown in Matrigel Overall design: Microarray analysis of RNA isolated from MCF7 cells transfected with CEACAM1-S wild-type (SW) or CEACAM1-S double-A mutant T457A,S459A (DA) grown in Matrigel for 4 days

参与管腔形成的基因鉴定：对在基质胶（Matrigel）中培养的、分别转染野生型CEACAM1-4S与T457A、S459A突变型CEACAM1-4S的MCF7细胞提取的信使RNA（mRNA）开展基因芯片分析。
实验设计概述：对在基质胶（Matrigel）中培养4天的MCF7细胞进行微阵列分析，这些细胞分别转染了CEACAM1-S野生型（SW）或CEACAM1-S双丙氨酸突变体T457A、S459A（DA）。