EGAS00001002599-sc-2020-11-19T16:44:20Z - samples

We devised an approach to disentangle the TCR and CD28 pathways upon stimulation in naive and memory primary human CD4+ T cells (Tcons) in response to defined stimulatory signals. Isolated memory and naïve T cells were activated using anti-CD3, anti-CD28 or both in combination. As a control we cultured cells in the same conditions but without the stimuli. We carried Chipmentation using the H3K27ac antibody on 200,000 cross-linked cells. 1) This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute please see http://www.sanger.ac.uk/datasharing/ .EGA dataset EGAD00001006611

我们开发了一种研究方法，用于解析在明确刺激信号作用下，初始型与记忆型原代人CD4阳性T细胞（Tcons）受刺激时的T细胞受体（TCR）与CD28信号通路。我们通过抗CD3抗体、抗CD28抗体或二者联合处理，对分离获得的记忆型与初始型T细胞进行激活。设置对照组：在完全相同的培养条件下，不添加任何刺激物培养细胞。我们针对20万个交联细胞，使用H3K27ac抗体开展染色质免疫沉淀标记测序（Chipmentation）实验。
1) 本数据属于预发布数据集。关于威康信托桑格研究所（Wellcome Trust Sanger Institute）共享的预发布数据的规范使用说明，请参阅http://www.sanger.ac.uk/datasharing/。该数据集编号为EGAD00001006611。