L2_A-class_neurons_tiling_array

modENCODE_submission_469 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: NC694 (engineered, target gene unc-119 tagged by 3xFlag::PAB-1); Tissue: L2-A-class; Developmental Stage: larva mid-L2 20dC 22h post-L1; Genotype: unc-119(ed1) III; wdEx257. Sex: mixed Male and Hermaphrodite population; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Antibody EZview Red ANTI-FLAG® M2 Affinity Gel (target is FLAG); Strain NC694 (engineered, target gene unc-119 tagged by 3xFlag::PAB-1); temperature 20; Developmental Stage larva mid-L2 20dC 22h post-L1; Tissue L2-A-class

modENCODE_submission_469 本提交源自Robert Waterston的modENCODE项目。如需查看modENCODE项目完整列表，请访问http://www.genome.gov/26524648。项目目标：本实验旨在通过将RNA与商用基因组拼接芯片（genome tiling arrays）杂交，检测秀丽隐杆线虫（C. elegans）的全部转录本。为最大化检测特定细胞中低表达稀有转录本的概率，我们将从经荧光激活细胞分选（FACS）分离的特定胚胎细胞，以及通过mRNA标记法获取的胚胎后细胞中提取RNA。有关数据使用条款与条件，请参阅http://www.genome.gov/27528022 及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。关键词：转录本拼接芯片分析（Transcript tiling array analysis）。实验类型：转录本拼接芯片分析。生物来源：菌株：NC694（工程改造菌株，靶基因unc-119经3xFlag::PAB-1标记）；组织：L2-A级；发育阶段：L1幼虫蜕皮后于20℃培养22小时的中期L2幼虫；基因型：unc-119(ed1) III; wdEx257；性别：雌雄混合种群（雄性与雌雄同体个体）；重复次数：3次；实验因素：抗体：EZview Red ANTI-FLAG® M2亲和凝胶（靶标为FLAG标签）；菌株NC694（工程改造菌株，靶基因unc-119经3xFlag::PAB-1标记）；温度：20℃；发育阶段：L1幼虫蜕皮后于20℃培养22小时的中期L2幼虫；组织：L2-A级