Animal samples metagenome Raw sequence reads. viral metagenome

The giant panda (Ailuropoda melanoleuca) is a vulnerable mammal herbivore living wild in central China. Viral infection has become a potential threat to the health of the endangered giant pandas, but limited viral genetic information related to these animals is available in public database. We describe here the metagenomics-derived viral sequences found in samples, including feces, blood, nasopharyngeal secretion and five different tissues, collected from a wild diseased and healthy captive giant pandas in China. A library was then constructed using Nextera XT DNA Sample Preparation Kit (Illumina) and then sequenced using the Miseq Illumina platform with 250 bases paired ends with a distinct molecular tag for each pool.

大熊猫（Ailuropoda melanoleuca）是野外栖息于中国中部的易危植食性哺乳动物。病毒感染已成为威胁濒危大熊猫健康的潜在风险之一，但目前公共数据库中针对该物种的病毒遗传信息仍较为匮乏。本研究采集了中国境内患病野生与健康圈养大熊猫的多种样本，包括粪便、血液、鼻咽分泌物及5种不同组织，并从中获取了宏基因组学来源的病毒序列。随后使用Illumina公司的Nextera XT DNA样本制备试剂盒构建测序文库，并通过Illumina MiSeq平台进行测序，采用250碱基双端测序策略，且每个混合样本池均带有独特的分子标签。