Comparison of mouse placental labyrinth and human villus tree. Comparison of mouse placental labyrinth and human villus tree

An important question for the use of the mouse as a model for studying human disease is the degree of functional conservation of genetic control pathways from human to mouse. The human placenta and mouse placenta show structural similarities but there has been no systematic attempt to assess their molecular similarities or differences. We built a comprehensive database of protein and microarray data for the highly vascular exchange region micro-dissected from the human and mouse placenta near-term. Abnormalities in this region are associated with two of the most common and serious complications of human pregnancy, maternal preeclampsia (PE) and fetal intrauterine growth restriction (IUGR), each disorder affecting ~5% of all pregnancies. To compare the gene expression patterns in the vascular exchange regions of the human (villus tree) and mouse (labyrinth) placenta. Keywords: comparison Overall design: Mouse labyrinth tissue was micro-dissected form naturally mated crosses of C57Bl/6J mice. Placentas were individually dissected on embryonic day 17.5. From each litter ¼ of the tissue were set aside for RNA extraction and microarray analysis and ¾ for cellular fractionation and proteomic analysis, as recently described (Kislinger et al., 2006). Human villous trees were dissected from term normal placenta delivered by cesarean section from a term pregnancy (~ 38 weeks). Tissue was divided for organellar fractionation and RNA extraction.

以小鼠作为人类疾病研究模型的关键问题之一，在于遗传调控通路从人类到小鼠的功能保守程度。人类胎盘与小鼠胎盘虽具备结构相似性，但此前尚无系统性研究对二者的分子相似性与差异进行评估。我们构建了一套涵盖蛋白质组与微阵列（microarray）数据的综合数据库，样本取自人类与小鼠足月胎盘的高血管化交换区域，该区域经显微切割获取。该区域的异常与两种最常见且严重的人类妊娠并发症密切相关：母体子痫前期（preeclampsia, PE）与胎儿宫内生长受限（intrauterine growth restriction, IUGR），两类疾病各影响约5%的妊娠人群。本研究旨在比较人类（绒毛树）与小鼠（迷路区）胎盘血管交换区域的基因表达模式。
关键词：比较研究
整体实验设计：小鼠迷路区（labyrinth）组织取自C57Bl/6J小鼠自然交配繁殖的后代，于胚胎第17.5天对胎盘进行单独解剖。每窝胎盘中，取1/4组织用于RNA提取与微阵列分析，剩余3/4用于细胞分级分离与蛋白质组（proteomic）分析，实验方法参照已发表文献（Kislinger等，2006）。人类绒毛树（villus tree）组织取自足月（约38周）剖宫产（cesarean section）分娩的正常胎盘，经解剖获取。获取的组织将被分为两份，分别用于细胞器分级分离与RNA提取。