The synthetic opioid fentanyl elicited transcriptomic alterations related to viral replication, immune response, and inflammation in vitro.. The synthetic opioid fentanyl elicited transcriptomic alterations related to viral replication, immune response, and inflammation in vitro.

The US is in the midst of a major drug epidemic fueled in large part by the widespread recreational use of syntheticopioids such as fentanyl. we evaluated the impact of fentanyl using in cell lines that replicate infectious viruses. RNAseq identified a number of hepatocyte genes that were differentially regulated by fentanyl, including those related to apoptosis, the antiviral / interferon response, chemokine signaling, and NFkB signaling. Overall design: Huh7.5 and HepG2 cell lines were exposed to fentanyl or left as untreated controls in duplicate.

美国正处于一场重大药物滥用流行病之中，该疫情主要由芬太尼(fentanyl)等合成阿片类药物(synthetic opioids)的广泛娱乐性使用所引发。本研究利用可支持感染性病毒复制的细胞系，评估了芬太尼的生物学影响。通过RNA测序(RNA-seq)分析，鉴定出一批受芬太尼差异调控的肝细胞基因，其中涉及细胞凋亡(apoptosis)、抗病毒/干扰素应答、趋化因子信号通路以及NFκB信号通路等相关生物学过程。实验设计概况：将Huh7.5与HepG2细胞系分为芬太尼处理组与未处理对照组，每组均设置两个生物学重复。