<b>Split-APEX implicates splicing factor SRSF1 and splicing helicases in ribosomal biogenesis</b>

Scaffold files following mass spectrometry of proteins isolated on streptavidin beads following proximity biotinylation. This was done using (1 file) full-length APEX2 fused to the N-terminus of SRSF1 and (1 file) split-APEX, with complementary portions of APEX2 fused to the N-terminus of SRSF1 and to the N-terminus of helicases involved in pre-mRNA splicing. Experiments were done in triplicate.本数据集包含的支架文件基于如下流程生成：先对样品进行邻近生物素化（proximity biotinylation），随后通过链霉亲和素磁珠（streptavidin beads）分离得到目标蛋白质，再对分离所得的蛋白质进行质谱（mass spectrometry）分析。本次实验采用两种实验体系，分别对应1个数据文件：其一为与SRSF1的N端融合的全长APEX2；其二为分裂型APEX2（split-APEX），即将APEX2的两个互补结构域分别融合至SRSF1的N端，以及参与前mRNA剪接（pre-mRNA splicing）的解旋酶（helicases）的N端。所有实验均设置三次生物学重复。