Study of PRMT5 mediated H3R2me2s in Th1-like iTregs. Study of PRMT5 mediated H3R2me2s in Th1-like iTregs

In this study, we have studied the genes associated with PRMT5 mediated histone methylation (H2R2me2s) in Th1-like iTregs. This particular epigenetic markers is upregulated in Th1 cells, and Th1-like iTregs. Therefore, we wanted to identify the genes H3R2me2s is regulating in Th1-like iTregs as compared to iTregs or Th1 cells. The goal of this sequencing was to identify if H2R2me2s is important for increased suppressive capacity of Th1-like iTregs or not. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for symmetric histone arginine methylation (H3R2me2s) in Th1-like iTregs, iTregs and Th1 cells of C57BL\6 mice

本研究针对Th1样诱导性调节性T细胞（Th1-like induced regulatory T cells，Th1-like iTregs）中与蛋白质精氨酸甲基转移酶5（Protein arginine methyltransferase 5，PRMT5）介导的组蛋白甲基化（H2R2me2s）相关的基因展开了研究。该表观遗传标记在辅助性T细胞1（T helper 1 cells，Th1）与Th1样诱导性调节性T细胞中均呈上调表达。因此，本研究旨在对比诱导性调节性T细胞（induced regulatory T cells，iTregs）、辅助性T细胞1与Th1样诱导性调节性T细胞，筛选出H3R2me2s在Th1样诱导性调节性T细胞中调控的靶基因。本次测序的研究目标为明确H2R2me2s是否对Th1样诱导性调节性T细胞的抑制功能增强具有关键作用。
整体实验设计：针对C57BL/6小鼠的Th1样诱导性调节性T细胞、诱导性调节性T细胞及辅助性T细胞1，开展组蛋白精氨酸对称甲基化（symmetric histone arginine methylation，H3R2me2s）的染色质免疫沉淀测序（Chromatin immunoprecipitation DNA-sequencing，ChIP-seq）