RNA Sequencing profiling of cells from wild tpye mESC and differentiated cells derived from mESCs, and brains from Wild Type and Discn-/- mice

Aim: The goals of this study are to compare transcriptome profile of mESCs and differentiated cells, and transcriptome profile of brains from wild type and Discn-/- mice. Overall design: mESCs and differentiated cells derived from mESCs were treated with 10 µM etoposide or 2 mM Hydroxyurea for 4 hours. Brains were isolated from 5 weeks old wild type (WT) and Discn-/- mice. RNA was isolated and RNA-seq was performed.

研究目的：本研究旨在对比小鼠胚胎干细胞（mouse embryonic stem cells，mESCs）与分化细胞的转录组谱，以及野生型（wild type，WT）与Discn-/-小鼠脑部的转录组谱。实验设计概述：将小鼠胚胎干细胞（mESCs）及其分化所得细胞分别用10 μM依托泊苷（etoposide）或2 mM羟基脲（Hydroxyurea）处理4小时。从5周龄野生型（WT）及Discn-/-小鼠体内分离脑组织。提取总RNA并进行RNA测序（RNA-seq）。