Symbiotic lifestyle of segmented filamentous bacteria (SFB) revealed by single-cell genomics: SFB-2.. Candidatus Arthromitus sp. SFB-2

Isolation of five individual segmented filamentous bacteria (SFB, Candidatus Arthromitus, Clostridiaceae) filaments from a fecal sample from a BALB/c SFB-monocolonized mouse on a microfluidic chip using laser-tweezers. Cells were lyzed, their genomes amplified, and sequenced. Assemblies were obtained for each individual SFB metagenome, and a co-assembly was obtained based on combining the reads from the five individual metagenomes.

本数据集的实验构建过程如下：利用激光光镊（laser-tweezers）在微流控芯片（microfluidic chip）上，从单一定殖分段丝状细菌（Segmented Filamentous Bacteria, SFB，候选节丝杆菌属（Candidatus Arthromitus），梭菌科（Clostridiaceae））的BALB/c小鼠粪便样本中，分离得到5个独立的分段丝状细菌丝状体。随后对分离获得的菌体进行裂解、基因组扩增与测序：分别完成每株独立SFB宏基因组（metagenome）的序列组装，并将5株独立宏基因组的测序读段（reads）合并后进行共组装，最终获得共组装序列。