ORF3A expression and/or CLCC1 knockdown in 293T cells

293T cells expressing dCas9-KRAB and a control or CLCC1 targeting sgRNA were subjected to mRNA-seq (Lexogen Corral). Cells were either transiently transfected with an mCherry control expression vector or a SARS-CoV-2 ORF3A-HA expression vector.

表达dCas9-KRAB及对照或靶向CLCC1的单向导RNA（single guide RNA, sgRNA）的293T细胞，借助Lexogen Corral平台完成了信使RNA测序（mRNA-seq）。将该类细胞分别以mCherry对照表达载体，或新型冠状病毒（SARS-CoV-2）开放读码框3A（ORF3A）融合血凝素（hemagglutinin, HA）标签的表达载体进行瞬时转染。