Maternal and neonatal microbiome around birth

The aims of this prospective longitudinal case-control pilot-study were (1) to characterize the vaginal, uterine and placental microbiome of pregnant women with an uneventful pregnancy course and a term delivery via elective caesarean section, as well as (2) to evaluate the influence of the maternal on the neonatal microbiome. The first aim was achieved by collecting vaginal and rectal swabs for microbiome analysis at inclusion, followed by vaginal, rectal, uterine and placental swabs that were collected during the caesarean section. The second aim was achieved by microbiome analysis of rectal, oral/buccal, and skin swabs taken from the newborns delivered by the study participants. Sequencing was performed at the Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna under the project ID JMF-2111-02.

本前瞻性纵向病例对照预试验的研究目标分为两点：(1) 对妊娠过程顺利且经择期剖宫产完成足月分娩的孕妇，对其阴道、子宫及胎盘微生物组（microbiome）进行特征解析；(2) 评估母体微生物组对新生儿微生物组的影响。第一项目标通过以下流程完成：在受试者入组时采集阴道拭子与直肠拭子用于微生物组分析，随后在剖宫产手术术中采集阴道、直肠、子宫及胎盘拭子。第二项目标则通过对本研究参与者娩出的新生儿采集直肠、口腔/颊部及皮肤拭子并开展微生物组分析得以达成。测序工作由维也纳医科大学与维也纳大学联合微生物组平台完成，项目编号为JMF-2111-02。