Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors [Ecoli]

We found that cyanobacterial RNA polymerase possesses very efficient intrinsic proofreading ability. This ability allows model species of cyanobacteria, Synechocystis sp PCC 6803 to keep in vivo level of transcriptional mistakes close to that of E.coli. Sequencing of total RNA prepared from exponentially grown cells

我们发现，蓝细菌的RNA聚合酶（RNA polymerase）拥有极为高效的内在校对能力。该能力可使蓝细菌模式物种集胞藻（Synechocystis sp PCC 6803）体内的转录错误水平维持在与大肠杆菌（E. coli）相近的水平。对从指数生长期细胞中制备的总RNA进行测序