Eubacterium rectale microbial mediated detoxification mechanism increases resilience of gut environment through removal of ROS and dampening innate immune response

High detection of Lachnospiraceae members were identified in dysbiotic IL-10 KO mice displaying similar levels of inflammation to control mice. Lachnospiraceae are a highly diverse family of microbes that produce short chain fatty acids, amino acids and antioxidants that may facilitate inflammation relief. Using molecular techniques, five Lachnospiraceae strains were investigated for lowering cellular inflammation and ROS levels. Cell free spent media (CFSM) from <i>Eubacterium rectale</i> resulted in lower ROS, and nitric oxide levels in stressed colon cells. Bioinformatic approaches combined with molecular techniques revealed in <i>E. rectale</i> the potential function of biosynthesis of an antioxidant, glutathione (GSH), and was confirmed using metabolomics on the cell free spent media of <i>E. rectale</i>. To investigate GSH potential for alleviating ROS damage, RNA sequencing of ROS damaged colon cells revealed downregulation of cell stress and immune response genes indicating promotion of recovery and its ability to alleviate ROS stress.<br>

研究人员在菌群失调的白细胞介素10敲除（IL-10 KO）小鼠中检出大量毛螺菌科（Lachnospiraceae）成员，该类小鼠的炎症水平与对照组小鼠相当。
毛螺菌科是一类高度多样的微生物类群，可合成短链脂肪酸、氨基酸与抗氧化物质，或可缓解炎症反应。
本研究通过分子技术，针对5株毛螺菌科菌株展开了降低细胞炎症与活性氧（ROS，Reactive Oxygen Species）水平的相关探究。
直肠真杆菌（Eubacterium rectale）的无细胞上清液（CFSM）可降低受胁迫结肠细胞中的活性氧与一氧化氮水平。
结合生物信息学与分子技术，本研究揭示了直肠真杆菌具备合成抗氧化物质谷胱甘肽（GSH）的潜在功能，并通过对其无细胞上清液的代谢组学分析验证了该结论。
为探究谷胱甘肽缓解活性氧损伤的潜力，本研究对受活性氧损伤的结肠细胞进行RNA测序，结果显示细胞应激与免疫应答相关基因的表达量下调，表明谷胱甘肽可促进细胞恢复，并具备缓解活性氧应激的能力。