Circadian and circatidal rhythms of protein abundance in the California mussel (Mytilus californianus)

Coastal habitats fluctuate with the 12.4 h tidal and 24 h light/dark cycle to predictably alter conditions such as air exposure, temperature, and food availability. Intertidal sessile bivalves exhibit behavioral and physiological adjustments to minimize the challenges of this environment. We investigated a high-resolution time course of the changes in protein abundance in the gill tissue of the intertidal mussel Mytilus californianus in a simulated tidal environment of 12:12 h light:dark cycles and a matching 6:6 h high:low tide cycle within each 12 h period. Approximately 38% of detected proteins showed significant rhythms in their abundances, with diversity in the phases of rhythmic isoforms. The circadian rhythm was dominant in protein abundance changes, particularly with oxidative metabolism. A tidal cycle elicited changes within functional groups, including in cytoskeletal proteins, chaperones, and oxidative stress proteins. In addition to protein abundance changes, we found the po...

海岸生境随12.4小时潮汐周期及24小时光暗周期波动，可预测性地改变空气暴露、温度及食物可获得性等条件。潮间带固着双壳类（intertidal sessile bivalves）通过行为与生理调节以最小化该环境带来的挑战。我们在模拟潮汐环境中（每12小时周期内包含12:12小时光暗循环及匹配的6:6小时高低潮循环），研究了潮间带贻贝加州贻贝（Mytilus californianus）鳃组织蛋白质丰度变化的高分辨率时间序列。约38%的检测蛋白质显示出显著的丰度节律，且节律亚型的相位具有多样性。昼夜节律（circadian rhythm）在蛋白质丰度变化中占主导地位，尤其与氧化代谢相关。潮汐周期引发功能组内的变化，包括细胞骨架蛋白、分子伴侣及氧化应激蛋白。除蛋白质丰度变化外，我们还发现po...