Association of serum biomarkers with post-thrombolytic symptomatic intracranial hemorrhage in stroke: A comprehensive protein microarray analysis from INTRECIS study

The study consecutively and prospectively collected blood samples from stroke patients in the INTRECIS study to investigate biomarkers associated with symptomatic intracranial hemorrhage after intravenous thrombolysis through comprehensively screening 49 pre-set biomarkers in microarray protein analysis. In the present study, we found baseline serum levels of CRP, GDNF, IFN-γ, IGFBP-6, IL-4, LYVE-1, MMP-2, PAI-1, and PDGF-AA were associated with post-thrombolytic symptomatic intracranial hemorrhage in acute ischemic stroke. Furthermore, PDGF-AA, IGFBP-6, and LYVE-1 were firstly found associated with symptomatic intracranial hemorrhage.

本研究依托INTRECIS研究，前瞻性连续收集卒中患者的血液样本，通过蛋白质微阵列分析全面筛查49种预设生物标志物，以探究静脉溶栓后症状性颅内出血相关的生物标志物。在本研究中，我们发现急性缺血性卒中患者的基线血清C反应蛋白(CRP)、胶质细胞源性神经营养因子(GDNF)、干扰素γ(IFN-γ)、胰岛素样生长因子结合蛋白6(IGFBP-6)、白细胞介素4(IL-4)、淋巴管内皮细胞透明质酸受体1(LYVE-1)、基质金属蛋白酶2(MMP-2)、纤溶酶原激活物抑制剂1(PAI-1)及血小板衍生生长因子AA(PDGF-AA)水平与溶栓后症状性颅内出血存在关联。此外，本研究首次发现PDGF-AA、IGFBP-6及LYVE-1与症状性颅内出血存在关联。