RNA sequencing analysis of HCT116 cells following 24 hours of metabolic recovery from methionine restriction +/- small molecule treatment.

We determined small molecule inhibition of H3K9 HMTs G9a/GLP during methionine restriction results in lasting changes in transcriptional profiles following metabolic recovery. Overall design: Examination of transcript profiles before and after metabolic recovery from methionine restriction +/- small molecule treatment.

我们证实，在甲硫氨酸限制（methionine restriction）期间对H3K9组蛋白甲基转移酶（H3K9 Histone Methyltransferase, HMTs）G9a/GLP实施小分子抑制，可在代谢恢复后对转录谱（transcriptional profiles）产生持久改变。整体实验设计：分析经甲硫氨酸限制联合/不联合小分子处理的样本，在代谢恢复前后的转录谱变化。