Studies of autophagy pathway during submergence in Arabidopsis thaliana. Studies of autophagy pathway during submergence in Arabidopsis thaliana

Autophagy involves massive degradation of intracellular components and functions as a conserved system that helps cells to adapt to adverse conditions. In Arabidopsis thaliana, submergence induces the transcription of autophagy-related (ATG) genes and the formation of autophagosomes. To study the role of autophagy during submergence, we performed transcriptome analysis with atg5, an autophagy-defective mutant, under submergence conditions. Our data showed that submergence changed the expression profile of DEG in the atg5 versus wild-type. Overall design: Whole Arabidopsis plants (4-week-old) were gently submerged at depths of 10 cm beneath the water surface under normal light/dark cycle condition. Plant samples for microarray were collected after 0 h or 48 h submergence treatment.

细胞自噬（Autophagy）指胞内组分的大规模降解，是一类保守的细胞调控系统，可帮助细胞适应不良环境。在拟南芥（Arabidopsis thaliana）中，淹水处理可诱导自噬相关（autophagy-related, ATG）基因的转录与自噬体（autophagosomes）的形成。为探究淹水处理过程中细胞自噬的功能，我们以自噬缺陷突变体atg5为实验材料，在淹水条件下开展了转录组分析。本研究数据显示，淹水处理改变了atg5与野生型植株间差异表达基因（differentially expressed genes, DEG）的表达谱。实验设计概述：将4周龄的完整拟南芥植株置于正常光暗循环条件下，于水面下10 cm深度处进行温和淹水处理；分别在淹水处理0 h和48 h时收集用于基因芯片检测的植物样本。