Bulk RNA-seq of T cells from EMT6 tumors treated with aTGFb/aPD-L1. Bulk RNA-seq of T cells from EMT6 tumors treated with aTGFb/aPD-L1

We performed bulk RNA sequencing on sorted T cells (7AAD- Calcein blue+ CD45+ THY1.1- TCRb+) in an orthotopic EMT6 tumor model 7 days after treatment initiation in four experimental groups: 1) Control 2) aPD-L1 3) aTGFb 4) aPD-L1 and aTGFb.

本研究对四个实验组的原位EMT6肿瘤模型，在治疗启动后7天时，对其中分选得到的表型为7AAD阴性、Calcein blue阳性、CD45阳性、THY1.1阴性、TCRβ阳性的T细胞开展了批量RNA测序（bulk RNA sequencing）。四个实验组具体包括：1) 对照组；2) 抗PD-L1（aPD-L1）治疗组；3) 抗TGFβ（aTGFb）治疗组；4) 抗PD-L1联合抗TGFβ治疗组。