Next Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Polyadenylated RNA

Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. We have made widespread use of Illumina sequencing technologies for RNA quantitation in several publications involving mouse hearts, dating from 2010, and wish to share both high-quality raw sequencing data and data processed to quantitate mRNA abundances from wild-type mice, male and female, at a variety of ages. These data will provide a resource for investigators using microarrays to understand the concentration of transcripts of interest relative to other cardiac RNAs, and will permit deeper interpretation of previous microarray studies. 40 cardiac polyA+-RNA profiles of 4- to 16 week-old FVB/NJ wild type (WT) mice were generated on Illumina HiSeq 2000 instruments.

研究目的：下一代测序（Next-generation sequencing，NGS）技术可实现RNA丰度定量，并能对组织与细胞内的RNA丰度进行对比，这是以往微阵列（microarray）技术无法实现的。自2010年起，我们已在多项针对小鼠心脏的研究中广泛应用Illumina测序技术开展RNA定量工作，现计划共享两类数据：一是高质量的原始测序数据，二是经处理后可定量不同年龄、雌雄野生型（wild-type，WT）小鼠心肌组织mRNA丰度的加工数据。本数据集将为使用微阵列技术的研究者提供参考资源，帮助其明确目标转录本相对于其他心肌RNA的表达水平，同时也可助力对既往微阵列研究进行更深入的解读。本数据集包含40份来自4至16周龄FVB/NJ野生型小鼠的心脏polyA+ RNA测序谱，测序平台为Illumina HiSeq 2000。