Raw data supporting "mScarlet fluorescence lifetime reports lysosomal pH quantitatively"

Original dataset and processing code supporting the preprint (scientific publication) "mScarlet fluorescence lifetime reports lysosomal pH quantitatively."

Publication Abstract: The lysosome maintains a highly acidic pH, which is critical for successful lysosomal catabolism. Lysosomal pH (pHlys) is difficult to measure because of the simultaneous need for a sensor with large dynamic range, genetic targetability, low pKa, and a quantitative readout. Here, we demonstrate that the fluorescence lifetime of the mScarlet-LAMP1 fusion protein quantitatively reports lysosomal pH, exhibiting a large dynamic range and a pKa well-tuned for the lysosome. Because fluorescence lifetime is an intrinsic property, pH measurements can be achieved in a single fluorescence channel. mScarlet-LAMP1 lifetime allows for individual lysosome-resolved recordings, a critical advance in describing and understanding pHlys heterogeneity. Using this biosensor, we quantify heterogeneity of pHlys in cultured cells at rest and over time during drug treatment. We anticipate that mScarlet-LAMP1 will enable new insights into the diversity of lysosomal physiology and ionic milieu.

支持预印本（科学出版物）《mScarlet荧光寿命定量报告溶酶体pH值》的原始数据集与处理代码。

论文摘要：溶酶体维持着极强的酸性pH环境，这对溶酶体高效完成分解代谢至关重要。由于同时需要具备宽动态范围、可遗传靶向性、低pKa值以及定量读出能力的传感器，溶酶体pH（pHlys）的检测颇具挑战。本研究证实，mScarlet-LAMP1融合蛋白的荧光寿命可定量反映溶酶体pH，其具备宽动态范围，且pKa值经过优化适配溶酶体环境。由于荧光寿命是一种固有属性，因此仅需单个荧光通道即可完成pH检测。mScarlet-LAMP1的荧光寿命可实现单溶酶体分辨率的记录，这为描述和解析pHlys的异质性带来了关键突破。借助该生物传感器，我们对静息状态下培养细胞中的pHlys异质性以及药物处理过程中的动态变化进行了定量分析。我们预计，mScarlet-LAMP1将为深入解析溶酶体生理功能与离子微环境的多样性提供新的研究视角。