Transcriptomic analysis by AmpliSeq Ion Torrent to study the involvement of CXXC5 in the regulation of TGFß/BMP target genes in glioblastoma

The aim of this work was to elucidate the role of the transcription factor CXXC5 in glioblastoma and study its involvement in the regulation of TGFß and BMP target genes in this context. To this end we used the patient derived glioblastoma stem cells U3034MG that were either transfected with siRNA targeting CXXC5 or with a non-targeting pool (as control) followed by stimulation with either 5ng/ml TGFß or 30ng/ml BMP7 for 24 h. Then we analysed how silencing CXXC5 affects the expression of TGFß and/or BMP7 target genes using Ion torrent AmpliseqTM.

本研究旨在阐明转录因子CXXC5在胶质母细胞瘤中的作用，并探究其在该病理背景下对转化生长因子β（TGF-β）及骨形态发生蛋白（BMP）靶基因的调控机制。为此，我们选用患者来源的胶质母细胞瘤干细胞U3034MG，将其分别转染靶向CXXC5的小干扰RNA（siRNA），或转染非靶向siRNA混合池作为对照；随后分别以5ng/ml的TGF-β或30ng/ml的BMP7刺激24小时。随后，我们借助Ion Torrent Ampliseq™ 测序技术，分析沉默CXXC5对TGF-β及/或BMP7靶基因表达的影响。