F1 P. vindemmiae progeny recovered from factorial crosses with Arsenophonus infected and uninfected mother and father wasps

Female P. vindemmiae wasps with and without Arsenophonus were crossed to males with and without Arsenophonus in a full factorial design, and then permitted to lay on D. melanogaster pupae. The number of flies emerging were recorded alongside the sex and number of F1 wasp progeny. One or two wasps per replicate were then tested for Arsenophonus presence by PCR. In addition, Wolbachia presence was also tested. <br> Column A: Treatment code (each colour represents a different cross type). Column B: Replicate within cross type. Column C: Cross type. First set is the female, second male. A+ = Arsenophonus +ve W+ = Wolbachia positive. A- =Arsenophonus -ve W- Wolbachia -ve. Column D: # flies emerging within 48 hours (measure of unparasitized fly pupae). <br> Mother data: Column E: QC PCR outcome for mother. + = good DNA, - = poor DNA Column F: PCR assay for mother for Wolbachia outcome: += present, -= absent Column G: PCR assay for mother for Arsenophonus outcome test 1: += present, -= absent Column H: PCR assay for mother for Arsenophonus test 2 outcome (HS PCR): += present, -= absent Column I: Conclusion concerning mother Arsenophonus/Wolbachia status from data in columns E-H. A+ = Arsenophonus +ve W+ = Wolbachia positive. A- =Arsenophonus -ve W- Wolbachia -ve. <br> Brood data: Column J: # wasp offspring emerging. Column K: # flies surviving Column L: # female wasps emerging Column M: #male wasps emerging. Column N: % male wasps in brood (males/total) Column O: # parasitized flies where wasp did not appear Column P: #Flies that died Column Q: fate unknown <br> Individual F1 progeny infection status: Offspring 1: Column R: sex of wasp Column S: QC status of DNA (+ = pass, - = fail) Column T: PCR assay for Wolbachia (+= present, - = absent) Column U: PCR assay for Arsenophonus (+= present, - = absent) Column V: Conclusion for infection status of wasp one (A+ = Arsenophonus present, W+= Wolbachia present). <br> Offspring 2 Column W: sex of wasp Column X: QC status of DNA (+ = pass, - = fail) Column Y: PCR assay for Wolbachia (+= present, - = absent) Column Z: PCR assay for Arsenophonus (+= present, - = absent) Column AA: Conclusion for infection status of wasp two (A+ = Arsenophonus present, W+= Wolbachia present). <br> <br>

本实验采用完全析因设计，将携带与不携带阿塞邦氏菌（Arsenophonus）的*P. vindemmiae*雌性寄生蜂，与携带或不携带阿塞邦氏菌的雄性寄生蜂进行交配，随后使其在黑腹果蝇（D. melanogaster）蛹上产卵。记录羽化的果蝇数量，并同步统计F1代寄生蜂子代的性别与数量。随后对每个重复组中的1或2头寄生蜂，通过聚合酶链式反应（PCR）检测其阿塞邦氏菌携带情况，同时额外检测沃尔巴克氏体（Wolbachia）的携带状态。

A列：处理组编码（每种颜色代表一种不同的交配组合类型）。
B列：同一交配组合类型内的重复组编号。
C列：交配组合类型，首位为母本，次位为父本。A+代表携带阿塞邦氏菌，W+代表携带沃尔巴克氏体；A-代表无阿塞邦氏菌，W-代表无沃尔巴克氏体。
D列：48小时内羽化的果蝇数量（反映未被寄生的果蝇蛹数量）。

母本数据：
E列：母本的PCR质量控制结果，+代表DNA质量合格，-代表DNA质量不合格。
F列：母本沃尔巴克氏体PCR检测结果，+代表检出，-代表未检出。
G列：母本阿塞邦氏菌首次PCR检测结果，+代表检出，-代表未检出。
H列：母本阿塞邦氏菌第二次PCR检测（高灵敏度聚合酶链式反应，HS PCR）结果，+代表检出，-代表未检出。
I列：基于E-H列数据得出的母本阿塞邦氏菌与沃尔巴克氏体携带状态结论，格式同C列：A+代表携带阿塞邦氏菌，W+代表携带沃尔巴克氏体；A-代表无阿塞邦氏菌，W-代表无沃尔巴克氏体。

子代蜂群数据：
J列：羽化的寄生蜂子代数量。
K列：存活的果蝇数量。
L列：羽化的雌性寄生蜂数量。
M列：羽化的雄性寄生蜂数量。
N列：蜂群中雄性寄生蜂占比（雄性数/总子代数）。
O列：被寄生但未发现寄生蜂子代的果蝇数量。
P列：死亡的果蝇数量。
Q列：结局未知（无法明确其最终状态）。

单头F1子代感染状态：
子代1：
R列：寄生蜂性别。
S列：DNA质量控制状态，+代表检测合格，-代表检测不合格。
T列：沃尔巴克氏体PCR检测结果，+代表检出，-代表未检出。
U列：阿塞邦氏菌PCR检测结果，+代表检出，-代表未检出。
V列：第1头子代寄生蜂的感染状态结论，格式为：A+代表携带阿塞邦氏菌，W+代表携带沃尔巴克氏体。

子代2：
W列：寄生蜂性别。
X列：DNA质量控制状态，+代表检测合格，-代表检测不合格。
Y列：沃尔巴克氏体PCR检测结果，+代表检出，-代表未检出。
Z列：阿塞邦氏菌PCR检测结果，+代表检出，-代表未检出。
AA列：第2头子代寄生蜂的感染状态结论，格式为：A+代表携带阿塞邦氏菌，W+代表携带沃尔巴克氏体。