Gene expression in pre-cystic mouse kidneys after Pkd2 deletion.

To identify early transcriptional changes downstream of Pkd2 mutations that promote cyst formation, we used tamoxifen-inducible CAG-Cre to induce Pkd2 deletion at postnatal day 2 and compared to wild-type mice at postnatal day 6 and 10. We then performed gene expression profiling analysis using data obtained from RNA-seq of whole mouse kidneys. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Pkd2 deleted mouse kidneys vs wild-type.

为鉴定Pkd2突变下游促进囊肿形成的早期转录改变，我们采用他莫昔芬诱导型CAG-Cre在小鼠出生后第2天诱导Pkd2基因敲除，并以出生后第6天和第10天的野生型小鼠作为对照。随后，我们通过完整小鼠肾脏的RNA测序（RNA-seq）数据开展基因表达谱分析。总体实验设计：针对Pkd2基因敲除小鼠肾脏与野生型小鼠肾脏的RNA-seq数据开展对比性基因表达谱分析。