TP53 loss initiates chromosomal instability in fallopian tube epithelial cells

High-grade serous ovarian cancer originates in the fallopian tube and is characterized by ubiquitous mutations in TP53. Here, we generated TP53 single-, TP53/BRCA1 and TP53/MYC double- and TP53/BRCA1/MYC triple-mutant subclones of the fallopian tube-derived cell line FNE1 using CRISPR/Cas9. These mutant subclones were subsequently subjected to RNA sequencing to determine the impact of these oncogenic mutations on signaling pathways. Overall design: RNA sequencing of 27 fallopian tube-derived cell lines.

高级别浆液性卵巢癌（High-grade serous ovarian cancer）起源于输卵管，其标志性特征为TP53基因的广泛突变。本研究通过CRISPR/Cas9技术，构建了输卵管来源细胞系FNE1的TP53单突变、TP53/BRCA1与TP53/MYC双突变，以及TP53/BRCA1/MYC三突变亚克隆株。随后对上述突变亚克隆株进行RNA测序，以探究这些致癌突变对细胞信号通路的调控影响。实验整体设计：对27株输卵管来源细胞系开展RNA测序。