Transcriptional profiling of atmbd4, atmbd6 and atmbd11 mutants of Arabidopsis thaliana. Arabidopsis thaliana

Methyl CpG Binding (MBD) proteins are a class of protein that binds with methylated DNA. In the model plant Arabidopsis thaliana there are 13 MBD proteins are there. To understand the gene regulation we performed microarray analysis of mutants of three genes (atmbd4, atmbd6 and atmbd11). Ten day old seedlings of these three mutant were compared with the wild type plants grown in same condition. Overall design: Surface sterilized Arabidopsis seeds were grown in MS media. Ten days old seedlings were used for isolation of RNA. The wild type plants along with T-DNA mutants of atmbd4, atmbd6 and atmbd11 were used to isolate the RNA. Three biological replicates of each sample were analyzed representing wild type, atmbd4, atmbd6 and atmbd11 of Arabidopsis.

甲基CpG结合（Methyl CpG Binding, MBD）蛋白是一类能够结合甲基化DNA的蛋白质。在模式植物拟南芥（Arabidopsis thaliana）中，共存在13种MBD蛋白。为解析基因调控机制，本研究对3个基因（atmbd4、atmbd6及atmbd11）的突变体开展了基因芯片分析（microarray analysis），将生长于相同培养条件下的这3个突变体的10日龄幼苗与野生型植株进行对照比较。
实验设计概述：将经表面灭菌的拟南芥种子接种于MS培养基（MS media）中培养，选取生长10天的幼苗用于RNA提取，所用材料包括野生型植株以及atmbd4、atmbd6、atmbd11的T-DNA插入突变体。每个样本设置3次生物学重复，涵盖拟南芥野生型、atmbd4、atmbd6及atmbd11四个组别。