Grass Carp diet and environmental data from the Lake Erie and Lake Michigan basins from 2019 to 2022

Data include metabarcoding read data from grass carp associated with the Great Lakes removal program from 2019 to 2022. The anterior portion of the digestive tract was carefully separated from other organs for each captured grass carp and stored in 180 proof non-denatured ethanol. A 25 ml of subsampled gut contents was used for DNA purification and sequencing using polymerase chain reactions. Samples were aligned with a custom ITS2 reference sequence database generated by downloading available ribosomal sequences from the NCBI Nucleotide database on February 5th, 2023. Algae were excluded from the database due to issues in extracting DNA from algae and minimal representation of cyanobacteria in DNA databases. Environmental covariates, such as discharge, number of road crossings, distance to cities, Lake Erie water levels, and temperature were collated based on the capture date of grass carp to assess relationships between diet and the environment. The data set includes grass carp capture and diet information and associated environmental variables. Short reads used for bioinformatic comparison obtained from the carp diets were deposited in the short read archive (SRA) associated with Bioproject PRJNA1304957, SRA Accessions SAMN50579620 - SAMN50580151.

本数据集涵盖2019至2022年参与五大湖清除项目的草鱼的元条形码（metabarcoding）测序读段数据。针对每尾捕获的草鱼，研究人员将其消化道前段与其余器官精细分离，随后以180 proof非变性乙醇保存。取25 mL肠道内容物子样本开展DNA纯化及聚合酶链式反应（polymerase chain reaction, PCR）测序工作。将测序样本与2023年2月5日从NCBI核苷酸数据库（NCBI Nucleotide database）下载核糖体序列构建的定制化内部转录间隔区2（ITS2）参考序列数据库进行比对。鉴于藻类DNA提取存在技术难点，且DNA数据库中蓝藻（cyanobacteria）的占比极低，研究团队将藻类从该参考数据库中剔除。基于草鱼的捕获日期，整理得到流量、道路跨度数、距城市距离、伊利湖水位及水温等环境协变量，以评估草鱼饮食与周边环境之间的关联。本数据集包含草鱼捕获信息、饮食信息及相关环境变量数据。用于生物信息学比对的草鱼饮食短读段数据，已提交至与生物项目PRJNA1304957相关的短读段归档（Short Read Archive, SRA），存档编号为SAMN50579620至SAMN50580151。