scRNA-seq analysis revealed the molecular characteristics of erythroid cells in spleen upon HAPC

High-altitude polycythemia (HAPC) is a most common chronic mountain sickness, characterized by excessive erythrocytosis caused primarily by persistent arterial hypoxia and ventilatory impairment. It has been a serious public health problem in high-altitude regions. Despite numerous studies on HAPC via genomics, transcriptomics or metabolome approaches, the pathogenesis of HAPC remains largely unclear. Here, we performed single-cell RNA sequencing to reveal the change of transcriptomics profiles of MNC in spleen of mice between HAPC subjects and healthy controls. Overall design: Single-cell RNA sequencing for MNC in spleen of mice between HAPC models and healthy controls.

高原红细胞增多症（High-altitude polycythemia, HAPC）是最常见的慢性高原病，以主要由持续性动脉低氧血症与通气功能障碍所致的红细胞过度增生为特征，已成为高原地区严重的公共卫生问题。尽管目前已有诸多通过基因组学、转录组学或代谢组学手段开展的HAPC相关研究，但其发病机制仍未完全阐明。本研究通过单细胞RNA测序技术，解析了高原红细胞增多症模型小鼠与健康对照组小鼠脾脏单个核细胞（mononuclear cells, MNC）的转录组谱变化。研究设计概况：针对高原红细胞增多症模型小鼠与健康对照组小鼠脾脏单个核细胞开展单细胞RNA测序。