MYCN knock-down leads to DNA-repair deficiency in human neuroblastoma (ChIP-Seq)

We knock down MYCN in human neuroblastoma cell line by RNAi. MYCN knock-down caused global epigenome change, including nucleosome gain on key DNA-repair genes and promoter H3K9ac down regulation. ChIP-seq of 6 histone modifications in ctrl/N-myc_kd neuroblastoma cell line BE2C

本研究通过RNA干扰（RNAi）技术在人类神经母细胞瘤细胞系中敲低MYCN基因。MYCN基因敲低可引发全基因组表观组改变，具体包括关键DNA修复基因区域的核小体富集，以及启动子区域H3K9乙酰化（H3K9ac）水平下调。本研究针对对照组（ctrl）与MYCN敲低组（N-myc_kd）的神经母细胞瘤细胞系BE2C，开展了6种组蛋白修饰的染色质免疫沉淀测序（ChIP-seq）检测。