CIL:37329

The video shows a cytoplast that is adhering to a sub-stratum in its central portion. It is also connected by a retraction fiber to its central cell. The cytoplast is engaging in vigorous protrusive activity around its periphery. The small lamellipodia is undergoing ruffling--extensions, folding back, and re-entry of the the protrusion into the main process of the cell. This demonstrates that an anucleate fragment of a cell has the capacity to nucleate actin, engage in ruffling, and retrograde flows. CIL 37336 and 37337 show that these fragments have the capacity to undergo directional locomotion. Video made available by Mark Cooper thru Zebrafish - The Living Laboratory.

本视频展示了一个胞质体（cytoplast），其中心区域黏附于底物（substratum）。该胞质体通过收缩纤维与其中心细胞相连，且在外周区域呈现出活跃的突起活动。小型片状伪足（lamellipodia）正发生膜褶运动——即突起延伸、折返并重新嵌入细胞主体突起结构。上述现象证实，细胞的无核片段具备肌动蛋白（actin）成核、膜褶形成以及逆行流动的能力。CIL 37336与CIL 37337的相关研究表明，此类片段可实现定向运动。本视频由马克·库珀（Mark Cooper）通过“斑马鱼-活体实验室（Zebrafish - The Living Laboratory）”平台发布。