Endothelial LATS2 is a suppressor of bone marrow fibrosis. Endothelial LATS2 is a suppressor of bone marrow fibrosis

The skeletal system vasculature and specialized capillary subtypes regulate crucial elements of bone growth and regeneration. However, little is known about endothelial cell (EC) activation and plasticity during bone remodeling. Here, we demonstrate that the inactivation of the gene encoding Hippo kinase large tumor suppressor kinase 2 (Lats2) in ECs results in the development of a vascular defect in the metaphysis, which favours accumulation of hypertrophic chondrocytes and induces bone marrow fibrosis in the bone. Overall design: Examination of endothelial and bone stromal cells in Lats2iECKO and control mice. Two samples per condition.

骨骼系统脉管系统与特化毛细血管亚型调控骨骼生长与再生的关键进程。然而，目前对于骨重塑过程中内皮细胞（endothelial cell, EC）的活化与可塑性仍所知甚少。本研究证实，在内皮细胞中失活编码Hippo激酶大肿瘤抑制激酶2（Lats2）的基因，会导致干骺端出现脉管缺陷，该缺陷可促进肥大软骨细胞蓄积，并诱发骨髓纤维化。实验整体设计：对Lats2iECKO小鼠与对照小鼠的内皮细胞及骨基质细胞进行检测，每组设置2个样本。