A genetic cluster of multidrug-resistant Enterobacter cloacae complex ST78 harboring a plasmid containing blaVIM-1 and mcr-9 in the Netherlands

Carbapenemases produced by Enterobacterales are often encoded by genes on transferable plasmids and represent a major health care problem, especially if the plasmids contain additional antibiotic resistance genes. As part of the Dutch national surveillance, medical microbiological laboratories submit Enterobacterales isolates suspected of carbapenemase production to the National Institute for Public Health and the Environment for characterization. In this study, the objective was to study the molecular characteristics of a genetic cluster of Enterobacter cloacae complex isolates collected in the period between 2015-2020.

肠杆菌目（Enterobacterales）产生的碳青霉烯酶（Carbapenemases）通常由可转移质粒上的基因编码，是医疗卫生领域的重大公共卫生难题，尤其当该类质粒携带额外抗生素抗性基因时。作为荷兰国家监测计划的组成部分，医学微生物实验室会将疑似产碳青霉烯酶的肠杆菌目分离株提交至荷兰公共卫生与环境研究所进行特征鉴定。本研究旨在对2015年至2020年间收集的阴沟肠杆菌复合群（Enterobacter cloacae complex）分离株的遗传簇开展分子特征分析。