Metabolic rates of the corals Montastrea cavernosa and Porites astreoides in response to experimental temperature variations (20-28ºC)@en

This dataset includes metabolic rates of the coral species Porites astreoides and Montastraea cavernosa from Bermuda that were exposed to a temperature reduction experiment for 37 days. Prior to the experiment, 8 corals were collected with a hammer and chisel on 16th June 2019 in 5 m depth at Sea Venture Shoals, Bermuda [32º22'53” N, 64º38'11” W], which is an exposed reef featuring a typical Bermuda reef community. Water temperature during collection was ~27°C. The seasonal temperature range of Bermuda reefs is ~19°C to 29°C. The laboratory-based experiment took place at the Bermuda Institute of Ocean Sciences from 1st July to 6th August 2019 and used flow-through seawater from the adjacent Reach. Light was provided by Indoor grow lights, Sun Blaze T5 Fluorescent 44. The successive treatments were: (i) constant control temperature at 28°C, and (ii) temperature reduction (0.5 °C/day) followed by constant temperature for 20 days at 24 °C and (iii) temperature reduction (0.5 °C/day) followed by constant temperature for 20 days at 20 °C. Metabolic rates were measured on day 1, 5, 9, 13, 17, 22, 26 and 37 of the experiment. Metabolic rates include photosynthesis and oxygen consumption rates measured by respirometry. Standardized to coral surface are net photosynthesis, respiration and gross photosynthesis. Metabolic rates were measured in 1 liter glass incubation chamber (kitchen ware) equipped with an oxygen sensor spot (OXSP5, PyroScience, Germany), a magnetic stirring bar, and a grid for coral placement above the stirring bar controlled by a magnetic stirring plate (LABOMAG Mulitpoint magnetic stirrer, SHP Steriltechnik, Germany). Light was supplied with aquarium lamps (PAR ∼210–240 μmol photons/m²/s; indoor grow light, Sun Blaze T5 Fluorescent 44). In addition, gross photosynthesis is standardized to zooxanthella cell, amount of light-harvesting/photo-collecting pigments and respiration is standardized to total biomass.

本数据集包含采自百慕大的两种珊瑚物种——星孔珊瑚（Porites astreoides）与巨形脑珊瑚（Montastraea cavernosa）的代谢速率数据，上述珊瑚参与了为期37天的降温实验。
实验前，研究人员于2019年6月16日在百慕大海湾冒险浅滩（Sea Venture Shoals，北纬32°22′53″，西经64°38′11″）的5米水深处，以铁锤和凿子采集了8株珊瑚；该区域为暴露型礁体，拥有典型的百慕大礁体群落。采集时水温约为27℃，百慕大礁体的季节水温范围约为19℃至29℃。
本实验室实验于2019年7月1日至8月6日在百慕大海洋科学研究所（Bermuda Institute of Ocean Sciences）开展，实验用水取自邻近的Reach海域的流通海水。光照由室内生长灯提供，型号为Sun Blaze T5 Fluorescent 44。
实验依次设置三组处理：
(i) 恒定对照温度组，水温维持28℃；
(ii) 降温组：以每日0.5℃的速率降温，随后在24℃下恒定维持20天；
(iii) 降温组：以每日0.5℃的速率降温，随后在20℃下恒定维持20天。
代谢速率分别在实验第1、5、9、13、17、22、26及37天完成测定。代谢速率涵盖通过呼吸计量法（respirometry）测定的光合速率与耗氧速率。其中净光合速率、呼吸速率及总光合速率均以珊瑚表面积为基准进行标准化。
代谢速率的测定在1升厨具级玻璃培养舱中进行，舱内配备氧气传感片（OXSP5，PyroScience，德国）、磁力搅拌子，以及用于将珊瑚置于搅拌子上方的网格架，该装置由LABOMAG Mulitpoint磁力搅拌器（SHP Steriltechnik，德国）控制。本次实验的光照由水族箱灯提供，光合有效辐射（Photosynthetically Active Radiation, PAR）约为210–240 μmol光子/m²/s，型号为Sun Blaze T5 Fluorescent 44室内生长灯。
此外，总光合速率额外以虫黄藻细胞数、光捕获/集光色素含量为基准进行标准化，呼吸速率则以总生物量为基准进行标准化。