Gene expression profiling of whole ovary in embryonic (gestational day 11, 12, 14, 16 & 18) and postnatal day 2 mice. (GUDMAP Series ID: 5)

The overall objective of this proposal is to map the temporal and spatial dynamics of gene expression in the fetal mouse testis at key developmental timepoints. Urogenital tract malformations are the most common birth defects in males and their incidence together with other male reproductive health concerns such as reduced fertility and testicular cancer are reportedly on the rise in the human population. To better understand the impact of genetic factors and environmental influences on testicular development, it is important to first understand normal gene expression patterns and signaling cascades within the fetal testis during development. The goal of this study is to identify cell-specific genes that can be used as biomarkers for key differentiation events. Keywords: developmental time course Day to day comparison in whole ovary throughout development with triplicates at each time point. Each sample is a pair of ovaries from a single animal and each animal is taken from a different dam (with the exception of gd11 sample numbers 18 and 19 which come from the same dam).本项目的总体目标为解析关键发育时间节点下小鼠胎儿睾丸内基因表达的时空动态特征。泌尿生殖道畸形是男性群体中最常见的出生缺陷，据报道，其发病率与人类种群中其他男性生殖健康问题（如生育能力下降、睾丸癌）均呈上升趋势。为深入阐明遗传因素与环境影响对睾丸发育的调控作用，首先明确发育过程中胎儿睾丸内的正常基因表达模式与信号级联反应至关重要。本研究的目标为鉴定可作为关键分化事件生物标志物的细胞特异性基因。关键词：发育时间序列；发育全程完整卵巢的逐日比对分析，每个时间点设置三次生物学重复。每份样本取自单只实验动物的一对卵巢，且每只动物均来自不同的母鼠（gd11样本的18号与19号除外，二者来自同一只母鼠）。