Proportions of different elements of secondary structure for GRFN-1 peptide in aqueous buffer, TFE-buffer and HFIP-buffer based on Circular Dichroic spectroscopic analysis.

*peptides in 10 mM phosphate buffer pH = 6.5, TFE:10 mM phosphate buffer pH = 6.5 (4∶6, v∶v) or HFIP:10 mM phosphate buffer pH = 6.5 (4∶6, v∶v) were analyzed for secondary conformation based on secondary structural analysis using Selcon (Methods).针对分别处于以下三种溶剂体系中的多肽，采用Selcon软件进行二级结构分析以解析其二级构象：10 mM pH 6.5磷酸盐缓冲液；体积比为4∶6的三氟乙醇（TFE）与10 mM pH 6.5磷酸盐缓冲液混合溶剂；以及体积比为4∶6的六氟异丙醇（HFIP）与10 mM pH 6.5磷酸盐缓冲液混合溶剂，相关方法详见方法部分。