Transcriptomic dysregulation of SDCCGA8 KO retinal epithelial and immature neuronal cells.. Transcriptomic dysregulation of SDCCGA8 KO retinal epithelial and immature neuronal cells.

RNA-seq transcirptomic profiling of SDCCAG8-deficient cells generated by gene-editing, identified differentially expressed genes that are enriched for in neurodevelopmental processes and extracellular matrix deposition and maintenance Overall design: RPE1 WT, RPE1 SDCCAG8 -/-, SH-SY5Y WT and SH-SY5Y SDCCAG8 -/- cells were sequenced in triplicate at baseline conditions and after serum starvation.

本数据集为基因编辑构建的SDCCAG8缺陷细胞的RNA测序（RNA-seq）转录组谱分析，所鉴定得到的差异表达基因显著富集于神经发育过程以及细胞外基质沉积与维持。
实验设计：在基础培养条件与血清饥饿处理条件下，分别对RPE1野生型（WT）、RPE1 SDCCAG8 -/-、SH-SY5Y野生型（WT）以及SH-SY5Y SDCCAG8 -/- 细胞进行三次生物学重复测序。