Effect of Evi-1 deletion in hematopoietic stem cells. Mus musculus

To identify the target genes of Evi-1 in hematopoietic stem cells (HSCs), we carried out genome-wide transcriptional analysis using wild-type and Evi-1-deleted HSCs. Overall design: Lineage(-), Sca-1(+), c-Kit(+) cells derived from Evi-1(flox/flox) mice were transduced with GFP or Cre-GFP expressing retroviruses. GFP(+) cells were sorted and and analyzed by Affymetrix® Mouse Genome 430 2.0 Array® for gene expression. Two independent experiments were performed.

为鉴定造血干细胞（hematopoietic stem cells, HSCs）中Evi-1的靶基因，我们采用野生型与Evi-1缺失的造血干细胞开展了全基因组转录组分析。实验整体设计：将源自Evi-1(flox/flox)小鼠的谱系阴性（Lineage(-)）、Sca-1阳性（Sca-1(+)）、c-Kit阳性（c-Kit(+)）细胞，使用表达绿色荧光蛋白（Green Fluorescent Protein, GFP）或Cre-GFP的逆转录病毒进行转导。随后分选GFP阳性细胞，通过Affymetrix® Mouse Genome 430 2.0 Array®开展基因表达分析。本研究共完成两次独立实验。