Sewage sludge stabilization methods alter soil microbes

We investigated how the stabilization process of sewage sludge determines its microbial communiy structure, and how this is reflected in the soil microbial community structure after land application. We pyrosequenced two phylogenetic markers (16S and 18S rRNA genes) to analyse the community structure of prokaryotes (bacteria+archaea) and fungi in two types of sewage sludge that had been produced under the same conditions but stabilized either through an autothermal thermophilic aerobic digestion (ATAD sludge) or a mesophilic anaerobic digestion (MAD sludge). We sequenced the same markers in agricultural soils either unamended (control soil), mineral fertilized (min fertil soil), amended with ATAD sludge (ATAD soil) or amended with MAD slduge (MAD soil). Five replicates were used in all cases. One representative sequence of each OTU is provided.

本研究探究了污泥稳定化工艺如何决定其微生物群落结构，以及该结构经农田土地施用后如何反映于土壤微生物群落结构之中。针对两种在相同条件下制备、但分别通过自热式高温好氧消化（autothermal thermophilic aerobic digestion, ATAD）和中温厌氧消化（mesophilic anaerobic digestion, MAD）实现稳定化的污泥，本研究选取16S和18S rRNA基因这两种系统发育标记物进行焦磷酸测序（pyrosequencing），以分析其中原核生物（细菌+古菌）与真菌的群落结构。同时对四类农田土壤开展相同标记物的测序：未施加任何改良剂的对照土壤、施加矿物肥料的土壤、施加ATAD稳定化污泥的土壤，以及施加MAD稳定化污泥的土壤。所有实验处理均设置5个生物学重复。本研究提供每个操作分类单元（Operational Taxonomic Unit, OTU）的一条代表性序列。