Pseudomonas aeruginosa CLJ1 genome sequencing, transcriptomics, and proteomics [transcriptomics]

Gene expression profiles of two Pseudomonas aeruginosa taxonomic outlier clinical isolates, CLJ1 and CLJ3 [CLJ1] Pseudomonas aeruginosa taxonomic outliers emerged recently as infectious for humans, provoking hemorrhagic pneumonia. Those bacteria lack classical type III secretion system, and utilize the pore-forming toxin for infection. Two clones CLJ1 and CLJ3 belonging to these taxonomic outliers have been isolated from the same patient at two different times during hospitalization. P. aeruginosa CLJ3 displays antibiotic resistance phenotype, while CLJ1 is more cytotoxic on epithelial and endothelial cells. Total RNA was isolated from CLJ1 and sequenced in duplicate using Illumina HiSeq to compare their genome-wide transcription levels and relate these to protein levels and genome sequence.

本数据集包含两株铜绿假单胞菌（Pseudomonas aeruginosa）分类学异常临床分离株CLJ1与CLJ3的基因表达谱。【CLJ1相关背景】近期发现铜绿假单胞菌分类学异常株可感染人类并诱发出血性肺炎，此类菌株缺乏经典Ⅲ型分泌系统（type III secretion system），而是通过成孔毒素（pore-forming toxin）介导感染过程。本研究从同一名住院患者的两个不同时间点，分离得到属于该类分类学异常株的两个克隆株CLJ1与CLJ3：其中铜绿假单胞菌CLJ3呈现抗生素耐药表型，而CLJ1对上皮细胞与内皮细胞具有更强的细胞毒性。研究者从CLJ1中提取总RNA，采用Illumina HiSeq平台进行双份重复测序，以比较二者全基因组范围内的转录水平，并将转录水平与蛋白质水平及基因组序列进行关联分析。