16S rRNA gene amplicons from nasal bovine swabs collected from apparently healthy and dairy calves diagnosed with Bovine Respiratory Disease in California.

The objective of the study focuses on the characterization of the nasal microbiome of apparently healthy (n=47) and BRD-affected (n=49) pen-mates dairy calves as a potential additional diagnostic tool to identify an animal with BRD. Samples were collected fom a farm in California. For the nasal microbiome analysis, the region V4 of the 16S rRNA gene was amplified and sequenced using Illumina MiSeq Sequencer.

本研究旨在对表观健康（n=47）与牛呼吸道疾病（BRD, Bovine Respiratory Disease）感染的同栏乳用犊牛的鼻腔菌群进行特征解析，以期将其作为鉴定BRD感染动物的潜在辅助诊断工具。样本采自加利福尼亚州的一处牧场。针对鼻腔菌群分析，研究人员对16S核糖体RNA（16S rRNA）基因的V4区进行扩增，并采用Illumina MiSeq测序仪完成测序。