Single cell sequencing: Capturing the origin and dynamics of chromosomal copy-number heterogeneity

Shallow whole-genome sequencing of single cells from individual clonal (patient-derived colorectal cancer) organoids was performed to investigate the degree of copy-number heterogeneity that emerges and propagates during clonal organoid outgrowth of a single cancerous cell. Selected single cells were sequenced with higher coverage. In addition, daughter cells were isolated to directly link mitotic chromatin errors to chromosomal copy number changes.EGA study EGAS00001003812

为探究单个癌细胞在其克隆类器官（clonal organoid）扩增过程中产生并传播的拷贝数异质性（copy-number heterogeneity）程度，本研究对来自单个患者来源结直肠癌克隆类器官的单细胞实施了浅层全基因组测序（shallow whole-genome sequencing）。研究筛选出部分单细胞，以更高测序覆盖度开展测序；此外还分离了子细胞，以直接将有丝分裂染色质异常（mitotic chromatin errors）与染色体拷贝数变化（chromosomal copy number changes）建立关联。该数据集对应EGA研究项目EGAS00001003812。