Summary of Trx redox status following TR1 knockdown and Sec-deficient TR1 expression: Percent Trx in each redox state (% total).

Summary of the 6 possible redox states, from the most oxidized (state 1) to the least (state 6) of Trx in RKO cells with endogenous TR1 (si-Scramble), with endogenous TR1 knocked-down (si-TR1), or endogenous TR1 knocked-down but with induced Sec-deficient mutated TR1 (si-TR1+mTR1). Since the majority of the Trx was found to be in the reduced state, we stimulated with 1 mM diamide for 30 min to oxidize the cells and those cells without endogenous TR1 display more oxidized Trx than cells with endogenous TR1.

本数据集总结了RKO细胞中硫氧还蛋白（Thioredoxin, Trx）的6种可能氧化还原状态，按氧化程度从高到低依次为状态1至状态6。实验涉及三类细胞：保留内源性硫氧还蛋白还原酶1（Thioredoxin Reductase 1, TR1）的阴性对照组（si-Scramble）、内源性TR1敲低组（si-TR1），以及内源性TR1敲低但诱导表达硒代半胱氨酸缺陷型突变TR1组（si-TR1+mTR1）。鉴于实验中已观测到绝大多数Trx处于还原状态，我们采用1 mM 二酰胺（diamide）刺激细胞30分钟以诱导细胞氧化。结果显示，缺失内源性TR1的细胞中Trx的氧化程度高于保留内源性TR1的细胞。