RNA-Seq analysis of melanoma cells, B16F10, in the presence of hypochlorous acid (HOCl). RNA-Seq analysis of melanoma cells, B16F10, in the presence of hypochlorous acid (HOCl)

We evaluated the changes in the transcriptome of a melanoma cell line, B16F10, and spleen isolated murine T cells when in the presence of hypochlorous acid (HOCl) using RNA-sequencing technologies. Overall design: mRNA profile changes comparing B16F10 cells without or in the presence of 600 uM HOCl and T cells without or in the presence of 600 uM HOCl

本研究采用RNA测序（RNA-sequencing）技术，检测黑色素瘤细胞系B16F10与脾脏分离的小鼠T细胞在次氯酸（hypochlorous acid, HOCl）处理下的转录组变化。整体实验设计：对比B16F10细胞在添加600 μM次氯酸与未添加条件下的mRNA表达谱变化，以及脾脏分离的小鼠T细胞在相同浓度次氯酸处理与未处理条件下的mRNA表达谱变化。