CRISPR and biochemical screens identify MAZ as a co-factor in CTCF-mediated insulation at Hox clusters [MAZ KO]

In this experiment, we sought to analyze how transcriptome of WT and MAZ knock-out (KO) cells change during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs) Overall design: Examination of gene expression of ESCs and cervical MNs using ribo-zero RNAseq, by knock-out status of MAZ in triplicates, 2 cell lines (WT and MAZ KO)

本实验旨在探究小鼠胚胎干细胞（mouse embryonic stem cells, ESCs）向颈段运动神经元（cervical motor neurons, MNs）分化过程中，野生型（Wild Type, WT）与MAZ基因敲除（knock-out, KO）细胞的转录组变化规律。整体实验设计：采用核糖体去除RNA测序（ribo-zero RNAseq）技术，针对2组细胞系（野生型与MAZ基因敲除型），按MAZ基因的敲除状态设置三次生物学重复，对胚胎干细胞及颈段运动神经元的基因表达水平进行检测。