Environmental DNA sequences: Murray River Channel 2009

Environmental 18S ribosomal RNA gene PCR amplicon sequence datasets and associated metadata. Contains Roche GS FLX 454 sequence data output files in *.sff format. Also includes *.csv files containing de-noised data processed using Amplicon Pyrosequence Denoising Program (APDP) and metadata describing the samples that were sequenced\nLineage: Micro-eukaryote biodiversity was assessed by pyrosequencing during summer and autumn at three sites that represent a flow gradient in the Murray River, Australia, towards the end of a ten year drought. Two sites (Hattah and Irrak) were in the river channel and one was a weir pool site (Mildura Weir Pool).\r\nWater samples and zooplankton net samples were collected from the open water, littoral zone and macrophytes at each site. The water samples were size fractionated at 2000, 500, 53, 1 and 0.22 µm. Pyrosequencing of the conserved 18S rRNA gene was used to detect eukaryotes\r\nWater samples were taken for phytoplankton identification, enumeration and chlorophyll a analysis. Measurements were made of incident photosynthetically active radiation, light attenuation, temperature and water flow. Metabolism rates were estimated from daily fluctuations in dissolved oxygen concentration in planktonic chambers in the river

环境18S核糖体RNA基因（18S ribosomal RNA gene）PCR扩增子序列数据集及相关元数据。包含以*.sff格式存储的Roche GS FLX 454测序数据输出文件；还包括*.csv格式文件，内含经扩增子焦磷酸测序降噪程序（Amplicon Pyrosequence Denoising Program, APDP）处理的降噪数据，以及描述所测序样本的元数据
谱系背景：在澳大利亚墨累河（Murray River）十年干旱末期的夏季与秋季，通过焦磷酸测序评估了微型真核生物（micro-eukaryote）的生物多样性。研究选取了代表该河流流量梯度的三个位点：其中两个（Hattah与Irrak）位于河道内，一个为堰塘位点（Mildura Weir Pool）。
在每个位点的开阔水域、沿岸带（littoral zone）及大型水生植物（macrophytes）生境中采集水样与浮游动物网样；水样按2000、500、53、1及0.22 µm的孔径进行粒径分级。通过保守的18S核糖体RNA基因焦磷酸测序检测真核生物
采集水样用于浮游植物鉴定、计数及叶绿素a分析；同时测定入射光合有效辐射（incident photosynthetically active radiation）、光衰减、温度与水流。通过河道内浮游生物室中溶解氧浓度的日波动估算代谢速率