Nacozari microbiomes and metagenomes. metagenome

We studied both culturable and metagenomic diversity or rhizospheric bacteria of mine tailing colonizing plants of Nacozari de García, Sonora, Mexico, through amplification of the 16S rRNA gene and whole metagenome shotgun sequencing. The culturable bacteria were assembled in a synthetic community (SC). Subsequently, we performed an experimental evolution experiment in the SC, selecting for heavy metal resistance, microbial competition, and growing in plant-derived food sources. The metabolic potential of the resulting final synthetic community (FSC) might present promising features that would make it useful for plant-growth promotion in a phytostabilization strategy.

本研究针对墨西哥索诺拉州纳科扎里·德·加西亚地区尾矿定殖植物的根际细菌，分别从可培养与宏基因组两个维度解析其物种多样性，分析手段涵盖16S rRNA基因扩增与全宏基因组鸟枪测序。研究人员将分离获得的可培养细菌构建为合成菌群（synthetic community, SC）；随后针对该合成菌群开展实验进化研究，筛选兼具重金属抗性、可在微生物竞争环境下定植且能利用植物源性营养基质生长的菌群成员。最终得到的终末合成菌群（final synthetic community, FSC）所具备的代谢潜能，有望展现出极具应用前景的特性，可用于植物稳定修复（phytostabilization）策略中的植物生长促进工作。