depth resolved crude oil spill aquifer and wetland sediments

The purpose of this study was to characterize the microbial functional and structural diversity with depth across representative redox zones of a hydrocarbon plume, and an adjacent uncontaminated wetland, at the Bemidji Oil Spill site. A combination of quantitative PCR, denaturing gradient gel electrophoresis, and pyrosequencing were applied to vertically sampled sediment cores.

本研究旨在表征贝米吉石油泄漏场地（Bemidji Oil Spill site）内，烃类羽流（hydrocarbon plume）的典型氧化还原带及其邻近未受污染湿地中，微生物功能与结构多样性随深度的分布特征。研究采用定量聚合酶链式反应（quantitative PCR）、变性梯度凝胶电泳（denaturing gradient gel electrophoresis）与焦磷酸测序（pyrosequencing）相结合的方法，对垂直采集的沉积物岩芯进行分析。