Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing [dCas9-DNMT3A ChIP-seq]

To investigate the genome-wide occupancy of the DNA methylation editing tool dCas9-DNMT3A in cells harboring non-targeting sgRNAs (CTRL) and cells harboring sgRNAs targeting the IL1RN promoter (sgIL1RN). Overall design: We analyzed the occupancy of dCas9-DNMT3A using chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq). We compared IL1RN methylation-edited cells (sgIL1RN) with non-edited cells (CTRL) using biological duplicates.

本研究旨在探究两类细胞中DNA甲基化编辑工具dCas9-DNMT3A的全基因组结合占据情况：一类为携带非靶向单引导RNA（single guide RNA，sgRNA）的对照细胞（CTRL组），另一类为携带靶向IL1RN基因启动子的单引导RNA（sgIL1RN）的实验组细胞（sgIL1RN组）。
整体实验设计：本研究采用染色质免疫共沉淀结合高通量测序（ChIP-seq）技术分析dCas9-DNMT3A的结合占据情况；通过设置生物学重复，对IL1RN甲基化编辑细胞（sgIL1RN组）与未编辑对照细胞（CTRL组）开展对比分析。