CIL:45860, Homo sapiens. In Cell Image Library

Plate 25566 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.

Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).

This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

编号25566的检测板：表型谱分析（Phenotypic profiling）旨在通过多参数、基于特征的分析手段，对每个样本的细胞表型进行系统性总结，使谱图间的相似性能够反映对应样本间的相似性。本图像集为测试基于图像的谱分析方法在区分小分子化合物作用效果方面的能力提供了研究基础。

图像
本图像集中的图像来源于经1600种已知生物活性化合物分别处理的U2OS细胞，并通过六种标记物标记七种细胞器（即细胞绘画（Cell Painting）检测实验）。

本先导实验共包含20块检测板。每块检测板设有384个孔，每个孔对应9个视场，总计69120个视场。每个视场通过5个通道（检测波长）进行成像，每个通道均存储为独立的灰度图像文件，因此共生成345600个16位TIFF格式的图像文件。